follicle development of xenotransplanted sheep ovarian tissue into male and female immunodeficient rats

نویسندگان

ghazaleh hajmusa

rouhollah fathi

mojtaba rezazadeh valojerdi

abdolhossein shahverdi

چکیده

background: this study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. we evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. materials and methods: in this experimental study, sheep ovarian cortical strips were transplanted into the neck back muscles of 8 male and 8 female immunodeficient, castrated rats. fourteen days after surgery, each rat was treated with human menopausal gonadotropin (hmg) for 9 weeks. one day after the last injection, ovarian tissues were removed and fixed for histology assessment. histology analyses were performed before and after grafting. estradiol (e2) levels were measured before and after gonadectomy, and at the end of the experiment. the control group consisted of 7 male and 7 female noncastrated/ non-grafted rats and the sham group comprised 7 male and 7 female castrated/ non-grafted rats for comparison of serum e2 concentrations. results: the percentage of primordial follicles decreased after transplantation in male (25.97%) and female (24.14%) rats compared to the control group (ovarian tissue nongrafted; 37.51%). preantral follicles increased in the male (19.5%) and female (19.49%) transplanted rats compared to the control group (11.4%). differences in antral follicles between male (0.06 ± 0.0%) and female (0.06 ± 0.0%) rats were not noticeable compared to control (1.25 ± 0.0%) rats. we observed a significantly higher percent of mean e2 secretion in grafted males compared to grafted females (p˂0.05). conclusion: despite significant differences in e2 secretion between xenografted male and female rats, we observed no statistical differences in terms of follicular development.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Follicle Development of Xenotransplanted Sheep Ovarian Tissue into Male and Female Immunodeficient Rats

Background This study aimed to assess follicle survival after xenotransplantation of sheep ovarian tissue into male and female immunodeficient rats. We evaluated the effects of gonadotropin treatment on follicular development in the transplanted tissue. MaterialsAndMethods In this experimental study, sheep ovarian cortical strips were transplanted into the neck back muscles of 8 male and 8 fema...

متن کامل

P-121: Follicle Development in Vitrified Sheep Ovarian Tissue Transplanted to Immunodeficiencent Rat

Background: Ovarian cortex xenotransplantation into immunodeficient rats is a follicles protection method in rare animals and investigation on folliclogenesis. The aim of this study was to evaluate the effects of gonadotropin and erythropoietin (EPO) treatment on follicular development in the transplanted ovaries. Materials and Methods: Prepared sheep ovarian cortical strips were vitrified by c...

متن کامل

I-6: Follicle Development in Culture of Frozen / Thawed Human Ovarian Tissue

The primary therapeutic goal for the oncology patient is survival. Recent advances in diagnoses and treatment of neoplasia have resulted in an ever-increasing number of patients being cured and resuming a normal life. However, recognized side effects of treatments used to eradicate malignancies are temporary or permanent sterility. The cryopreservation of ovarian tissue harvested before cancer ...

متن کامل

Effects of Subacute Administration of Co-Trimoxazole and Folic Acid on Ovarian Tissue in Adult Female Rats

Background: Previous studies have reported the antifertility activities of sulfonamides. This study was designed to evaluate the effects of co-trimoxazole and its co-administration with folic acid on ovarian tissue in female rats.Methods: A total of 54 rats were randomly divided into 9 groups (n=6). Group I served as the control and group II (vehicle) received saline. Other groups, III to IX, r...

متن کامل

Angiogenic factors and ovarian follicle development

Ovarian follicles require an adequate blood supply for oxygen, nutrients and hormones, in addition to eliminating CO2 and other metabolites. Acquisition of an adequate vascular supply is probably a limiting step in the selection and maturation of the dominant follicle. In this way, there is a progressive interest in the study of the growth factors involved in the angiogenic process. In addition...

متن کامل

Ovarian follicle development and transgenic mouse models.

Ovarian follicle development is a complex process that begins with the establishment of what is thought to be a finite pool of primordial follicles and culminates in either the atretic degradation of the follicle or the release of a mature oocyte for fertilization. This review highlights the many advances made in understanding these events using transgenic mouse models. Specifically, this revie...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of fertility and sterility

جلد ۹، شماره ۳، صفحات ۳۵۴-۳۶۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023